The Living Brain was a robot invented by International Computing Machines Corporation. At that time the Living Brain was considered the most sophisticated robot of that time and went around on a "good will tour", visiting schools and cities throughout America. Liz Allan and Peter Parker interacted with the robot when it arrived at Midtown High School for a demonstration of its abilities . It claimed to have deduced the identity of Spider-Man, but it's answer was never revealed. Later that night however two thieves tried steal the robot, yet they only managed to cause it to malfunction and wreak havoc. Spider-Man was informed of the chaos that the robot was causing and managed to stop it after a short scuffle.
Years later the Living Brain was rebuilt by a scientists son and sent on rampage. It again was opposed by Spider-Man, who again destroyed it.